Single Cell Isolation Microfluidics

In this work a microfluidic method that is capable of isolating single cells in droplets selecting successful edited clones and expansion of these isoclones is introduced.

Single cell isolation microfluidics.

Many single cell analysis techniques require the isolation of individual cells. Dielectrophoretic digital sorting enzymatic digestion facs hydrodynamic traps laser capture microdissection manual picking microfluidics micromanipulation serial dilution and raman tweezers. 131 132 in parallel this technology has been developed commercially by gigagen. Single cell analysis has become one of the most important applications of microfluidic technology in recent years.

Using a hybrid microfluidics method droplets in channels can be individually addressed using a co planar electrode system. Depending on the application single cell analysis chips can include any or all the above mentioned steps. We demonstrate the benefit of this approach to screen antibodies for the specific binding of cancer target cells in a high throughput single cell format which should have immense clinical potential. It refers to the process of isolation manipulation and analysis of the cells at single cell resolution in microfluidic chips.

133 134 abvitro later acquired by juno therapeutics developed a similar approach for sequencing. The throughput of this method was later increased to 10 6 cells by single cell isolation using droplet microfluidics instead of compartmentalization in microwells.