Single Cell Dna Methylation Sequencing

Single cell genome and transcriptome sequencing have revealed ith in several cancer types 8 12.

Single cell dna methylation sequencing.

Whole genome bisulphate methylc seq wgbs is the most comprehensive method for dname sequencing providing single base methylation profiles for more than 90 of the cpgs in the genome. Line joxm 1 an induced pluripotent stem cell ipsc line derived from fibroblasts cells from hipsci project was cultured and triggered into differentiation towards endoderm. Single cell dna methylome sequencing quantifies dna methylation. There are several known types of methylation that occur in nature including 5 methylcytosine 5mc 5 hydroymethylcytosine 5hmc 6 methyladenine 6ma and 4mc 4 methylcytosine 4mc.

It is essential for maintenance of cellular identity and is associated with a number of key processes including genomic imprinting x chromosome inactivation repression of transposable elements aging and carcinogenesis. Recent technological innovations have spurred the development of methods that enable us to study the occurrence and biology of this mark at the single cell level. The introduction of single cell dna methylation sequencing dna methylation is recognized as a principal contributor to the normal development and regulation of gene expression. It can also be used to establish composite methylomes that account for cell to cell heterogeneity in complex tissue samples.

Leveraging the power of next generation sequencing ngs both genome wide analysis and targeted approaches can provide researchers with insight into methylation patterns at a single nucleotide level. Dna methylation is an epigenetic modification that plays an important role in gene expression regulation development and disease. Benefits of methylation sequencing cytosine methylation can significantly modify temporal and spatial gene expression and chromatin remodeling. Here we performed single cell dna methylome sequencing for human preimplantation embryos and found that tens of thousands of genomic loci exhibited de novo dna methylation.

Dna methylation chromatin states and their interrelationships represent critical epigenetic information but these are largely unknown in human early embryos. Here we apply single cell chromatin overall omic scale landscape sequencing sccool seq to generate a genome wide map of dna methylation and chromatin accessibility at single cell resolution during human preimplantation development. Single cell transcription and methylation data was generated from a single donor from the human induced pluripotent stem cells initiative hipsci 15 16 using the previously described protocol for single cell methylation and transcriptome sequencing in the same cells scm t seq see for details.